Total protein determination

Introduction

 The determination of cell proteins through protein assays depends on technique and sensitivity of method, units’ definition, interfering compounds and removing substances interfering with the process before samples of the assay and correlating information from the techniques used.

Gel electrophoresis is used to separate and analyze molecules of protein and protein’s fragments on the basis of charge and seize. Bovine Serum Albumin is normally used in identifying the protein contained in a given fractions or cells and more so their concentration in a sample.  BSA is normally used as standard for protein concentration. As a standard it is used in the determination of quantities of various proteins, through a comparison of unknown protein quantity to some known amounts (In Neves & In Reis, 2016). Bradford Protein essay is used in measuring the protein concentration in a given serum or solution. It is quite fast, fairly accurate and allows samples that fall out of range to be retested within shortest time possible (In Neves & In Reis, 2016). The concentration of protein is determined through a comparison with a series of protein standards which can show linear absorbance profile in the assay. The Lowry assay is used to determine protein total level in a given solution and is normally exhibited by a change of sample solution color proportionately to protein concentration.

In the results for this experiment, there was concentration of BSA proteins is showed to be increasing as   from 0.048 up to 0.575 as the level of the reagent increases. This means that absorbance of the BSA was increasing as the protein concentration levels were rising. The limitations or errors arising from the essay include matrix interference by materials that are highly concentrated or high concentration of proteins.

Reference

In Neves, N. M., & In Reis, R. L. (2016). Biomaterials from nature for advanced devices and therapies.540